A “Second-Generation Process” for the Synthesis of l-Neopentylglycine: Asymmetric Reductive Amination Using a Recombinant Whole Cell Catalyst
A “second-generation process” for the enantioselective synthesis of l-neopentylglycine has been developed, realizing the first whole cell-catalyzed approach to this pharmaceutically interesting, bulky amino acid. The synthesis of this nonnatural amino acid proceeds highly efficiently via a reductive...
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Veröffentlicht in: | Organic process research & development 2006-05, Vol.10 (3), p.666-669 |
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Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
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Zusammenfassung: | A “second-generation process” for the enantioselective synthesis of l-neopentylglycine has been developed, realizing the first whole cell-catalyzed approach to this pharmaceutically interesting, bulky amino acid. The synthesis of this nonnatural amino acid proceeds highly efficiently via a reductive amination of the corresponding α-keto acid in the presence of a recombinant whole cell catalyst, bearing a leucine dehydrogenase and formate dehydrogenase. The desired l-neopentylglycine was obtained with >95% conversion and a high enantioselectivity of >99% ee at substrate concentrations of up to 88 g/L. |
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ISSN: | 1083-6160 1520-586X |
DOI: | 10.1021/op0501702 |