An Efficient and Economical MTT Assay for Determining the Antioxidant Activity of Plant Natural Product Extracts and Pure Compounds
Antioxidants scavenge free radicals, singlet oxygen, and electrons in cellular redox reactions. The yellow MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] is reduced to a purple formazan by mitochondrial enzymes. NADPH is the basis of established in vitro cell viability assays. A...
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| Veröffentlicht in: | Journal of natural products (Washington, D.C.) D.C.), 2010-07, Vol.73 (7), p.1193-1195 |
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| container_end_page | 1195 |
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| container_issue | 7 |
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| container_title | Journal of natural products (Washington, D.C.) |
| container_volume | 73 |
| creator | Liu, Yunbao Nair, Muraleedharan G |
| description | Antioxidants scavenge free radicals, singlet oxygen, and electrons in cellular redox reactions. The yellow MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] is reduced to a purple formazan by mitochondrial enzymes. NADPH is the basis of established in vitro cell viability assays. An antioxidant assay has been developed utilizing the redox reaction between MTT and selected natural product extracts and purified compounds. This simple, fast, and inexpensive MTT antioxidant assay is comparable with the lipid peroxidation inhibitory assay and can be mechanized to achieve high throughput. |
| doi_str_mv | 10.1021/np1000945 |
| format | Article |
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This simple, fast, and inexpensive MTT antioxidant assay is comparable with the lipid peroxidation inhibitory assay and can be mechanized to achieve high throughput.</description><identifier>ISSN: 0163-3864</identifier><identifier>EISSN: 1520-6025</identifier><identifier>DOI: 10.1021/np1000945</identifier><identifier>PMID: 20565070</identifier><identifier>CODEN: JNPRDF</identifier><language>eng</language><publisher>Northbrook, IL: American Chemical Society and American Society of Pharmacognosy</publisher><subject>Antioxidants - economics ; Antioxidants - metabolism ; Antioxidants - pharmacology ; Biological and medical sciences ; Coloring Agents - economics ; Coloring Agents - pharmacology ; Formazans ; Free Radical Scavengers - economics ; Free Radical Scavengers - metabolism ; Free Radical Scavengers - pharmacology ; General pharmacology ; Medical sciences ; Mitochondria - enzymology ; Molecular Structure ; NADP - metabolism ; Oxidation-Reduction ; Pharmacognosy. Homeopathy. 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Nat. Prod</addtitle><description>Antioxidants scavenge free radicals, singlet oxygen, and electrons in cellular redox reactions. The yellow MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] is reduced to a purple formazan by mitochondrial enzymes. NADPH is the basis of established in vitro cell viability assays. An antioxidant assay has been developed utilizing the redox reaction between MTT and selected natural product extracts and purified compounds. This simple, fast, and inexpensive MTT antioxidant assay is comparable with the lipid peroxidation inhibitory assay and can be mechanized to achieve high throughput.</description><subject>Antioxidants - economics</subject><subject>Antioxidants - metabolism</subject><subject>Antioxidants - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Coloring Agents - economics</subject><subject>Coloring Agents - pharmacology</subject><subject>Formazans</subject><subject>Free Radical Scavengers - economics</subject><subject>Free Radical Scavengers - metabolism</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>General pharmacology</subject><subject>Medical sciences</subject><subject>Mitochondria - enzymology</subject><subject>Molecular Structure</subject><subject>NADP - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - economics</subject><subject>Plant Extracts - metabolism</subject><subject>Plant Extracts - pharmacology</subject><subject>Singlet Oxygen - chemistry</subject><subject>Tetrazolium Salts - economics</subject><subject>Thiazoles - economics</subject><issn>0163-3864</issn><issn>1520-6025</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkD1PwzAQhi0EgvIx8AeQFwaGwNlJnGaMSvmQCnQoc-T4A1w1dmU7iM78cQIt7cItJ52ee0_3IHRO4JoAJTd2SQCgzPI9NCA5hYQBzffRAAhLk3TIsiN0HMK8Z1Io80N0RCFnORQwQF-VxWOtjTDKRsytxGPhrGuN4Av8NJvhKgS-wtp5fKui8q2xxr7h-K5wZaNxn0byfrES0XyYuMJO4-niZ_LMY-f7jKl3shMRjz-j5yKG3xvTzis8cu3SdVaGU3Sg-SKos00_Qa9349noIZm83D-OqknCMwIxkWTYV0MoI7KQXBSlVumw0SWRGZSFJjylkgDJS5kXEoQEJlnGUjlsiobSIj1BV-tc4V0IXul66U3L_aomUP-IrLcie_ZizS67plVyS_6Z64HLDcBD70p7boUJOy4FxqCkO46LUM9d523_4j8HvwGm9Yba</recordid><startdate>20100723</startdate><enddate>20100723</enddate><creator>Liu, Yunbao</creator><creator>Nair, Muraleedharan G</creator><general>American Chemical Society and American Society of Pharmacognosy</general><general>American Society of Pharmacognosy</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20100723</creationdate><title>An Efficient and Economical MTT Assay for Determining the Antioxidant Activity of Plant Natural Product Extracts and Pure Compounds</title><author>Liu, Yunbao ; Nair, Muraleedharan G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a410t-d18888b1261d7dac79fe38bf91d4097f1a32d10159d57d0cd06d6463d8b7b2273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Antioxidants - economics</topic><topic>Antioxidants - metabolism</topic><topic>Antioxidants - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Coloring Agents - economics</topic><topic>Coloring Agents - pharmacology</topic><topic>Formazans</topic><topic>Free Radical Scavengers - economics</topic><topic>Free Radical Scavengers - metabolism</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>General pharmacology</topic><topic>Medical sciences</topic><topic>Mitochondria - enzymology</topic><topic>Molecular Structure</topic><topic>NADP - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - economics</topic><topic>Plant Extracts - metabolism</topic><topic>Plant Extracts - pharmacology</topic><topic>Singlet Oxygen - chemistry</topic><topic>Tetrazolium Salts - economics</topic><topic>Thiazoles - economics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Yunbao</creatorcontrib><creatorcontrib>Nair, Muraleedharan G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of natural products (Washington, D.C.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Yunbao</au><au>Nair, Muraleedharan G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Efficient and Economical MTT Assay for Determining the Antioxidant Activity of Plant Natural Product Extracts and Pure Compounds</atitle><jtitle>Journal of natural products (Washington, D.C.)</jtitle><addtitle>J. Nat. Prod</addtitle><date>2010-07-23</date><risdate>2010</risdate><volume>73</volume><issue>7</issue><spage>1193</spage><epage>1195</epage><pages>1193-1195</pages><issn>0163-3864</issn><eissn>1520-6025</eissn><coden>JNPRDF</coden><abstract>Antioxidants scavenge free radicals, singlet oxygen, and electrons in cellular redox reactions. The yellow MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] is reduced to a purple formazan by mitochondrial enzymes. NADPH is the basis of established in vitro cell viability assays. An antioxidant assay has been developed utilizing the redox reaction between MTT and selected natural product extracts and purified compounds. This simple, fast, and inexpensive MTT antioxidant assay is comparable with the lipid peroxidation inhibitory assay and can be mechanized to achieve high throughput.</abstract><cop>Northbrook, IL</cop><pub>American Chemical Society and American Society of Pharmacognosy</pub><pmid>20565070</pmid><doi>10.1021/np1000945</doi><tpages>3</tpages></addata></record> |
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| identifier | ISSN: 0163-3864 |
| ispartof | Journal of natural products (Washington, D.C.), 2010-07, Vol.73 (7), p.1193-1195 |
| issn | 0163-3864 1520-6025 |
| language | eng |
| recordid | cdi_crossref_primary_10_1021_np1000945 |
| source | ACS Publications; MEDLINE |
| subjects | Antioxidants - economics Antioxidants - metabolism Antioxidants - pharmacology Biological and medical sciences Coloring Agents - economics Coloring Agents - pharmacology Formazans Free Radical Scavengers - economics Free Radical Scavengers - metabolism Free Radical Scavengers - pharmacology General pharmacology Medical sciences Mitochondria - enzymology Molecular Structure NADP - metabolism Oxidation-Reduction Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - economics Plant Extracts - metabolism Plant Extracts - pharmacology Singlet Oxygen - chemistry Tetrazolium Salts - economics Thiazoles - economics |
| title | An Efficient and Economical MTT Assay for Determining the Antioxidant Activity of Plant Natural Product Extracts and Pure Compounds |
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