An Efficient and Economical MTT Assay for Determining the Antioxidant Activity of Plant Natural Product Extracts and Pure Compounds

Antioxidants scavenge free radicals, singlet oxygen, and electrons in cellular redox reactions. The yellow MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] is reduced to a purple formazan by mitochondrial enzymes. NADPH is the basis of established in vitro cell viability assays. A...

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Veröffentlicht in:	Journal of natural products (Washington, D.C.) D.C.), 2010-07, Vol.73 (7), p.1193-1195
Hauptverfasser:	Liu, Yunbao, Nair, Muraleedharan G
Format:	Artikel
Sprache:	eng
Schlagworte:	Antioxidants - economics Antioxidants - metabolism Antioxidants - pharmacology Biological and medical sciences Coloring Agents - economics Coloring Agents - pharmacology Formazans Free Radical Scavengers - economics Free Radical Scavengers - metabolism Free Radical Scavengers - pharmacology General pharmacology Medical sciences Mitochondria - enzymology Molecular Structure NADP - metabolism Oxidation-Reduction Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - economics Plant Extracts - metabolism Plant Extracts - pharmacology Singlet Oxygen - chemistry Tetrazolium Salts - economics Thiazoles - economics
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Beschreibung
Zusammenfassung:	Antioxidants scavenge free radicals, singlet oxygen, and electrons in cellular redox reactions. The yellow MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] is reduced to a purple formazan by mitochondrial enzymes. NADPH is the basis of established in vitro cell viability assays. An antioxidant assay has been developed utilizing the redox reaction between MTT and selected natural product extracts and purified compounds. This simple, fast, and inexpensive MTT antioxidant assay is comparable with the lipid peroxidation inhibitory assay and can be mechanized to achieve high throughput.
ISSN:	0163-3864 1520-6025
DOI:	10.1021/np1000945