RNA Polymer Translocation with Single-Walled Carbon Nanotubes

RNA Polymer Translocation with Single-Walled Carbon Nanotubes

The translocation of single-walled carbon nanotubes (SWNTs) across MCF7 breast cancer cells was demonstrated with radioisotope labeling. Hybrids of SWNT−RNA polymer poly(rU) were formed through a nonspecific binding mechanism which could allow for the dissociation of the poly(rU) from the SWNTs upon...

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Veröffentlicht in:Nano letters 2004-12, Vol.4 (12), p.2473-2477
Hauptverfasser: Lu, Qi, Moore, Jessica M, Huang, George, Mount, Andrew S, Rao, Apparao M, Larcom, Lyndon L, Ke, Pu Chun
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Conformational dynamics in molecular biology
Cross-disciplinary physics: materials science
rheology
DNA, RNA
Dynamics and conformational changes
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Materials science
Molecular biophysics
Nanoscale materials and structures: fabrication and characterization
Nanotubes
Nucleic acids
Physics
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Zusammenfassung:The translocation of single-walled carbon nanotubes (SWNTs) across MCF7 breast cancer cells was demonstrated with radioisotope labeling. Hybrids of SWNT−RNA polymer poly(rU) were formed through a nonspecific binding mechanism which could allow for the dissociation of the poly(rU) from the SWNTs upon delivery. The cellular uptake of the hybrids was examined by confocal fluorescence microscopy. Through cell growth and MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assays, we showed the negligible cytotoxicity of SWNTs (up to 0.5 mg/mL) to MCF7 cells.
ISSN:1530-6984
1530-6992
DOI:10.1021/nl048326j