Resonance Raman Spectroscopy of Met121Glu Azurin

Resonance Raman Spectroscopy of Met121Glu Azurin

The resonance Raman spectra for Pseudomonas aeruginosa Met121Glu azurin have been measured at wavelengths throughout the 600-nm absorption band at low and high pH. The spectra of the mutant at pH 3.5 and 7.0 are identical. Analysis of the 600-nm absorption band and resulting resonance Raman excitati...

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Veröffentlicht in:The journal of physical chemistry. B 2000-11, Vol.104 (46), p.10915-10920
Hauptverfasser: Webb, M. Adam, Kiser, Cynthia N, Richards, John H, Di Bilio, Angel J, Gray, Harry B, Winkler, Jay R, Loppnow, Glen R
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Sprache:eng
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Zusammenfassung:The resonance Raman spectra for Pseudomonas aeruginosa Met121Glu azurin have been measured at wavelengths throughout the 600-nm absorption band at low and high pH. The spectra of the mutant at pH 3.5 and 7.0 are identical. Analysis of the 600-nm absorption band and resulting resonance Raman excitation profiles under both pH conditions indicates that the excited-state distortions in this mutant are smaller than those in the native protein.
ISSN:1520-6106
1520-5207
DOI:10.1021/jp000832j