Functional scFv Antibody Sequences against the Organophosphorus Pesticide Chlorpyrifos

Two functional single-chain Fv (scFv) antibodies that recognize specifically the widely used organophosphorus pesticide chlorpyrifos-ethyl were derived from two murine hybridoma cell lines. It is shown that the functional scFvs could be isolated without any rounds of selection, with a success rate d...

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Veröffentlicht in:	Journal of agricultural and food chemistry 2000-02, Vol.48 (2), p.335-337
Hauptverfasser:	Alcocer, Marcos J. C, Doyen, Christine, Lee, Heather A, Morgan, Michael R. A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Biological and medical sciences Biotechnology Chemical control Chlorpyrifos - immunology Control Fundamental and applied biological sciences. Psychology Genetic Engineering Genetic technics Immunoglobulin Fragments - genetics Immunoglobulin Fragments - immunology Immunoglobulin Variable Region - genetics Immunoglobulin Variable Region - immunology Insecticides - immunology Methods. Procedures. Technologies Modification of gene expression level Molecular Sequence Data Phytopathology. Animal pests. Plant and forest protection Protozoa. Invertebrates Sequence Alignment
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Zusammenfassung:	Two functional single-chain Fv (scFv) antibodies that recognize specifically the widely used organophosphorus pesticide chlorpyrifos-ethyl were derived from two murine hybridoma cell lines. It is shown that the functional scFvs could be isolated without any rounds of selection, with a success rate dependent on the efficiency of amplification of the functional light chain gene family by the specific primers. Besides four new functional immunoglobulin variable gene sequences, the isolation of a new pseudogene is reported. Keywords: Variable region; antibody engineering; immunoassay; recombinant antibody; antibody pseudogene
ISSN:	0021-8561 1520-5118
DOI:	10.1021/jf990690u