Partial Purification and Characterization of Sucrose Phosphate Synthase from Preclimacteric and Climacteric Bananas

Sucrose phosphate synthase (SPS) (EC 2.4.1.14) was purified from bananas (Musa acuminata cv. Nanicão) at different developmental and ripening stages (70, 110, and 130 days after anthesis), corresponding to tissue with different structure and composition. Banana SPS exists in three polymeric forms as...

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Veröffentlicht in:	Journal of agricultural and food chemistry 1997-04, Vol.45 (4), p.1103-1107
Hauptverfasser:	Nascimento, João R. O., Cordenunsi, Beatriz R., Lajolo, Franco M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Biological and medical sciences Economic plant physiology ENZIMAS ENZYME ENZYMES Fundamental and applied biological sciences. Psychology MUSA ACUMINATA Nutrition. Photosynthesis. Respiration. Metabolism PURIFICACION PURIFICATION
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Zusammenfassung:	Sucrose phosphate synthase (SPS) (EC 2.4.1.14) was purified from bananas (Musa acuminata cv. Nanicão) at different developmental and ripening stages (70, 110, and 130 days after anthesis), corresponding to tissue with different structure and composition. Banana SPS exists in three polymeric forms as observed by electrophoresis and immunoblotting, similar in all stages studied, with M w respectively of 180, 254, and 686 kDa. The three forms have the same 116 kDa subunit, and their activity increased during ripening associated with increased protein synthesis as shown by Western blotting. Keywords: Musa acuminata; banana; sucrose phosphate synthase; ripening; sucrose
ISSN:	0021-8561 1520-5118
DOI:	10.1021/jf960407g