Method for the Production and Characterization of Tomato Cutin Oligomers

A series of oligomeric fragments can be obtained by partial alkaline hydrolysis of cutin. The oligomers obtained from tomato cutin were analyzed by high-performance liquid chromatography-mass spectrometry and the major products were characterized as linear dimeric esters of omega,8-, omega,9-, and o...

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Veröffentlicht in:	Journal of agricultural and food chemistry 1995-08, Vol.43 (8), p.2134-2137
Hauptverfasser:	Osman, Stanley F, Gerard, Herve C, Fett, William F, Moreau, Robert A, Dudley, Robert L
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Agronomy. Soil science and plant productions BEURRE VEGETAL Biological and medical sciences Chemical constitution Economic plant physiology ESPECTROMETRIA DE MASAS ESTER ESTERASAS ESTERASE ESTERES ESTRUCTURA QUIMICA Fundamental and applied biological sciences. Psychology GRASAS VEGETALES HIDROLISIS HPLC HYDROLYSE LYCOPERSICON ESCULENTUM SPECTROMETRIE DE MASSE STRUCTURE CHIMIQUE
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Beschreibung
Zusammenfassung:	A series of oligomeric fragments can be obtained by partial alkaline hydrolysis of cutin. The oligomers obtained from tomato cutin were analyzed by high-performance liquid chromatography-mass spectrometry and the major products were characterized as linear dimeric esters of omega,8-, omega,9-, and omega,10-dihydroxyhexadecanoic acids, the major monomers of tomato cutin. Analysis of the products obtained from tomato cutin by treatment with cutinase suggests an exo mode of depolymerization for this enzyme
ISSN:	0021-8561 1520-5118
DOI:	10.1021/jf00056a033