Electrochemical Aptasensor Based on Proximity-Dependent Surface Hybridization Assay for Single-Step, Reusable, Sensitive Protein Detection

This study developed a novel electrochemical biosensor for single-step detection of proteins based on proximity-dependent surface hybridization assay. This strategy relied on simultaneous recognition of a target molecule by a pair of affinity probes, which was a prerequisite for efficiently promotin...

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Veröffentlicht in:Journal of the American Chemical Society 2007-12, Vol.129 (50), p.15448-15449
Hauptverfasser: Zhang, Yan-Li, Huang, Yong, Jiang, Jian-Hui, Shen, Guo-Li, Yu, Ru-Qin
Format: Artikel
Sprache:eng
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Zusammenfassung:This study developed a novel electrochemical biosensor for single-step detection of proteins based on proximity-dependent surface hybridization assay. This strategy relied on simultaneous recognition of a target molecule by a pair of affinity probes, which was a prerequisite for efficiently promoting the ferrocene-labeled tail sequences of the proximate affinity probe pair to hybridize together with surface-tethered oligonucleotide, thus triggering the redox current of ferrocene at the electrode. The strategy, as a universal methodology for developing high-performance biosensors, was demonstrated using an aptamer probe to a homodimer protein PDGF-BB, and the fabricated aptasensor showed intrinsic high sensitivity, excellent resistance to nonspecific interferences, and ready reusability.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja0773047