ENDOR of Superoxide Dismutase: Structure Determination of the Copper Site from Randomly Oriented Specimen

ENDOR of Superoxide Dismutase: Structure Determination of the Copper Site from Randomly Oriented Specimen

Using native and deuterated randomly-oriented frozen-solution specimens of copper-zinc SOD from human and bovine erythrocytes at about 5 K, ENDOR spectra were obtained. This has allowed for the reconstruction of the prosthetic group at the copper site in SOD, revealing that it is unchanged from that...

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Veröffentlicht in:Journal of Physical Chemistry 1994-09, Vol.98 (35), p.8806-8812
Hauptverfasser: Reinhard, Heiko, Kappl, Reinhard, Huettermann, Juergen, Viezzoli, Maria-Silvia
Format: Artikel
Sprache:eng
Schlagworte:
40 CHEMISTRY
COPPER
CRYSTAL STRUCTURE
ENDOR
HYDROGEN
LIGANDS
MATERIALS SCIENCE
NITROGEN 14
PHYSICS
SUPEROXIDE DISMUTASE
ZINC
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Zusammenfassung:Using native and deuterated randomly-oriented frozen-solution specimens of copper-zinc SOD from human and bovine erythrocytes at about 5 K, ENDOR spectra were obtained. This has allowed for the reconstruction of the prosthetic group at the copper site in SOD, revealing that it is unchanged from that obtained by X-ray analysis at room temperature. 14 refs., 8 figs., 4 tabs.
ISSN:0022-3654
1541-5740
DOI:10.1021/j100086a035