Well-Defined Poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) Vectors with Low Toxicity and High Gene Transfection Efficiency
Successful gene delivery vectors for clinical translation should have high transfection efficiency and minimal toxicity. In this work, well-defined poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) (PGEA) vectors with flanking cationic secondary amine and nonionic hydroxyl units were prepa...
Gespeichert in:
| Veröffentlicht in: | Biomacromolecules 2010-06, Vol.11 (6), p.1437-1442 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1442 |
|---|---|
| container_issue | 6 |
| container_start_page | 1437 |
| container_title | Biomacromolecules |
| container_volume | 11 |
| creator | Xu, F. J Chai, M. Y Li, W. B Ping, Y Tang, G. P Yang, W. T Ma, J Liu, F. S |
| description | Successful gene delivery vectors for clinical translation should have high transfection efficiency and minimal toxicity. In this work, well-defined poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) (PGEA) vectors with flanking cationic secondary amine and nonionic hydroxyl units were prepared via the ring-opening reaction of the pendant epoxide groups of poly(glycidyl methacrylate) with the amine moieties of ethanolamine. It was found that PGEA carriers possess very low toxicity ( |
| doi_str_mv | 10.1021/bm100309y |
| format | Article |
| fullrecord | <record><control><sourceid>acs_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1021_bm100309y</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>e13818600</sourcerecordid><originalsourceid>FETCH-LOGICAL-a344t-3391f05d2e989fe761aa1a8a54b6b2c485760d76bf60c05f0a0f50fe01581ed53</originalsourceid><addsrcrecordid>eNpt0EtPxCAQB3BiNL4PfgHDxcQ9oAMF2h7N-kw20cOqx4ZScDFt2UCN8g382NbnXjwxTH6ZyfwROqBwQoHR07qjABmUaQ1tU8Ek4RLY-lctSJ6X-RbaifEZAMqMi020xYAzyUFuo_dH07bk3FjXmwbf-TYdM7JITfBvqSUZWf3MsEit6lzvJ8vgl6nF3dhSOozdwUzwg9GDDxG_umGBZ_4Vz_2b025IWPUNvnZPC3xleoPnQfXRjtj5Hl9YOxrT67SHNqxqo9n_eXfR_eXFfHpNZrdXN9OzGVEZ5wPJspJaEA0zZVFak0uqFFWFEryWNdO8ELmEJpe1laBBWFBgBVgDVBTUNCLbRZPvuTr4GIOx1TK4ToVUUag-06z-0hzt4bddvtSdaf7kb3wjOPoBKmrV2vE07eLKsRIKRvnKKR2rZ_8S-vHEfxZ-ACPPigo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Well-Defined Poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) Vectors with Low Toxicity and High Gene Transfection Efficiency</title><source>MEDLINE</source><source>ACS Publications</source><creator>Xu, F. J ; Chai, M. Y ; Li, W. B ; Ping, Y ; Tang, G. P ; Yang, W. T ; Ma, J ; Liu, F. S</creator><creatorcontrib>Xu, F. J ; Chai, M. Y ; Li, W. B ; Ping, Y ; Tang, G. P ; Yang, W. T ; Ma, J ; Liu, F. S</creatorcontrib><description>Successful gene delivery vectors for clinical translation should have high transfection efficiency and minimal toxicity. In this work, well-defined poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) (PGEA) vectors with flanking cationic secondary amine and nonionic hydroxyl units were prepared via the ring-opening reaction of the pendant epoxide groups of poly(glycidyl methacrylate) with the amine moieties of ethanolamine. It was found that PGEA carriers possess very low toxicity (<10% of the toxicity of branched polyethylenimine (PEI, 25 kDa), while exhibiting surprisingly excellent transfection efficiency (higher than or comparable to that of PEI (25 kDa)) in different cell lines. A series of transfection and cytotoxicity assays revealed that PGEAs are highly promising as a new class of safe and efficient gene delivery vectors for future clinical gene therapies.</description><identifier>ISSN: 1525-7797</identifier><identifier>EISSN: 1526-4602</identifier><identifier>DOI: 10.1021/bm100309y</identifier><identifier>PMID: 20426406</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Applied sciences ; Biological and medical sciences ; Cell Culture Techniques ; Cell Line ; Cell Survival - drug effects ; Chemical modifications ; Chemical reactions and properties ; Cloning, Molecular ; DNA - administration & dosage ; DNA - genetics ; Drug Carriers - chemical synthesis ; Drug Carriers - chemistry ; Drug Carriers - toxicity ; Exact sciences and technology ; Gene Transfer Techniques ; General pharmacology ; Humans ; Luciferases - genetics ; Medical sciences ; Molecular Structure ; Organic polymers ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Physicochemistry of polymers ; Plasmids ; Polymethacrylic Acids - chemical synthesis ; Polymethacrylic Acids - chemistry ; Polymethacrylic Acids - toxicity ; Renilla - genetics ; Transfection</subject><ispartof>Biomacromolecules, 2010-06, Vol.11 (6), p.1437-1442</ispartof><rights>Copyright © 2010 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a344t-3391f05d2e989fe761aa1a8a54b6b2c485760d76bf60c05f0a0f50fe01581ed53</citedby><cites>FETCH-LOGICAL-a344t-3391f05d2e989fe761aa1a8a54b6b2c485760d76bf60c05f0a0f50fe01581ed53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bm100309y$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bm100309y$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22908214$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20426406$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, F. J</creatorcontrib><creatorcontrib>Chai, M. Y</creatorcontrib><creatorcontrib>Li, W. B</creatorcontrib><creatorcontrib>Ping, Y</creatorcontrib><creatorcontrib>Tang, G. P</creatorcontrib><creatorcontrib>Yang, W. T</creatorcontrib><creatorcontrib>Ma, J</creatorcontrib><creatorcontrib>Liu, F. S</creatorcontrib><title>Well-Defined Poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) Vectors with Low Toxicity and High Gene Transfection Efficiency</title><title>Biomacromolecules</title><addtitle>Biomacromolecules</addtitle><description>Successful gene delivery vectors for clinical translation should have high transfection efficiency and minimal toxicity. In this work, well-defined poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) (PGEA) vectors with flanking cationic secondary amine and nonionic hydroxyl units were prepared via the ring-opening reaction of the pendant epoxide groups of poly(glycidyl methacrylate) with the amine moieties of ethanolamine. It was found that PGEA carriers possess very low toxicity (<10% of the toxicity of branched polyethylenimine (PEI, 25 kDa), while exhibiting surprisingly excellent transfection efficiency (higher than or comparable to that of PEI (25 kDa)) in different cell lines. A series of transfection and cytotoxicity assays revealed that PGEAs are highly promising as a new class of safe and efficient gene delivery vectors for future clinical gene therapies.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Cell Culture Techniques</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Chemical modifications</subject><subject>Chemical reactions and properties</subject><subject>Cloning, Molecular</subject><subject>DNA - administration & dosage</subject><subject>DNA - genetics</subject><subject>Drug Carriers - chemical synthesis</subject><subject>Drug Carriers - chemistry</subject><subject>Drug Carriers - toxicity</subject><subject>Exact sciences and technology</subject><subject>Gene Transfer Techniques</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Luciferases - genetics</subject><subject>Medical sciences</subject><subject>Molecular Structure</subject><subject>Organic polymers</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Physicochemistry of polymers</subject><subject>Plasmids</subject><subject>Polymethacrylic Acids - chemical synthesis</subject><subject>Polymethacrylic Acids - chemistry</subject><subject>Polymethacrylic Acids - toxicity</subject><subject>Renilla - genetics</subject><subject>Transfection</subject><issn>1525-7797</issn><issn>1526-4602</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0EtPxCAQB3BiNL4PfgHDxcQ9oAMF2h7N-kw20cOqx4ZScDFt2UCN8g382NbnXjwxTH6ZyfwROqBwQoHR07qjABmUaQ1tU8Ek4RLY-lctSJ6X-RbaifEZAMqMi020xYAzyUFuo_dH07bk3FjXmwbf-TYdM7JITfBvqSUZWf3MsEit6lzvJ8vgl6nF3dhSOozdwUzwg9GDDxG_umGBZ_4Vz_2b025IWPUNvnZPC3xleoPnQfXRjtj5Hl9YOxrT67SHNqxqo9n_eXfR_eXFfHpNZrdXN9OzGVEZ5wPJspJaEA0zZVFak0uqFFWFEryWNdO8ELmEJpe1laBBWFBgBVgDVBTUNCLbRZPvuTr4GIOx1TK4ToVUUag-06z-0hzt4bddvtSdaf7kb3wjOPoBKmrV2vE07eLKsRIKRvnKKR2rZ_8S-vHEfxZ-ACPPigo</recordid><startdate>20100614</startdate><enddate>20100614</enddate><creator>Xu, F. J</creator><creator>Chai, M. Y</creator><creator>Li, W. B</creator><creator>Ping, Y</creator><creator>Tang, G. P</creator><creator>Yang, W. T</creator><creator>Ma, J</creator><creator>Liu, F. S</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20100614</creationdate><title>Well-Defined Poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) Vectors with Low Toxicity and High Gene Transfection Efficiency</title><author>Xu, F. J ; Chai, M. Y ; Li, W. B ; Ping, Y ; Tang, G. P ; Yang, W. T ; Ma, J ; Liu, F. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a344t-3391f05d2e989fe761aa1a8a54b6b2c485760d76bf60c05f0a0f50fe01581ed53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Cell Culture Techniques</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Chemical modifications</topic><topic>Chemical reactions and properties</topic><topic>Cloning, Molecular</topic><topic>DNA - administration & dosage</topic><topic>DNA - genetics</topic><topic>Drug Carriers - chemical synthesis</topic><topic>Drug Carriers - chemistry</topic><topic>Drug Carriers - toxicity</topic><topic>Exact sciences and technology</topic><topic>Gene Transfer Techniques</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Luciferases - genetics</topic><topic>Medical sciences</topic><topic>Molecular Structure</topic><topic>Organic polymers</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Physicochemistry of polymers</topic><topic>Plasmids</topic><topic>Polymethacrylic Acids - chemical synthesis</topic><topic>Polymethacrylic Acids - chemistry</topic><topic>Polymethacrylic Acids - toxicity</topic><topic>Renilla - genetics</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, F. J</creatorcontrib><creatorcontrib>Chai, M. Y</creatorcontrib><creatorcontrib>Li, W. B</creatorcontrib><creatorcontrib>Ping, Y</creatorcontrib><creatorcontrib>Tang, G. P</creatorcontrib><creatorcontrib>Yang, W. T</creatorcontrib><creatorcontrib>Ma, J</creatorcontrib><creatorcontrib>Liu, F. S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Biomacromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, F. J</au><au>Chai, M. Y</au><au>Li, W. B</au><au>Ping, Y</au><au>Tang, G. P</au><au>Yang, W. T</au><au>Ma, J</au><au>Liu, F. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Well-Defined Poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) Vectors with Low Toxicity and High Gene Transfection Efficiency</atitle><jtitle>Biomacromolecules</jtitle><addtitle>Biomacromolecules</addtitle><date>2010-06-14</date><risdate>2010</risdate><volume>11</volume><issue>6</issue><spage>1437</spage><epage>1442</epage><pages>1437-1442</pages><issn>1525-7797</issn><eissn>1526-4602</eissn><abstract>Successful gene delivery vectors for clinical translation should have high transfection efficiency and minimal toxicity. In this work, well-defined poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) (PGEA) vectors with flanking cationic secondary amine and nonionic hydroxyl units were prepared via the ring-opening reaction of the pendant epoxide groups of poly(glycidyl methacrylate) with the amine moieties of ethanolamine. It was found that PGEA carriers possess very low toxicity (<10% of the toxicity of branched polyethylenimine (PEI, 25 kDa), while exhibiting surprisingly excellent transfection efficiency (higher than or comparable to that of PEI (25 kDa)) in different cell lines. A series of transfection and cytotoxicity assays revealed that PGEAs are highly promising as a new class of safe and efficient gene delivery vectors for future clinical gene therapies.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>20426406</pmid><doi>10.1021/bm100309y</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1525-7797 |
| ispartof | Biomacromolecules, 2010-06, Vol.11 (6), p.1437-1442 |
| issn | 1525-7797 1526-4602 |
| language | eng |
| recordid | cdi_crossref_primary_10_1021_bm100309y |
| source | MEDLINE; ACS Publications |
| subjects | Animals Applied sciences Biological and medical sciences Cell Culture Techniques Cell Line Cell Survival - drug effects Chemical modifications Chemical reactions and properties Cloning, Molecular DNA - administration & dosage DNA - genetics Drug Carriers - chemical synthesis Drug Carriers - chemistry Drug Carriers - toxicity Exact sciences and technology Gene Transfer Techniques General pharmacology Humans Luciferases - genetics Medical sciences Molecular Structure Organic polymers Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Physicochemistry of polymers Plasmids Polymethacrylic Acids - chemical synthesis Polymethacrylic Acids - chemistry Polymethacrylic Acids - toxicity Renilla - genetics Transfection |
| title | Well-Defined Poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) Vectors with Low Toxicity and High Gene Transfection Efficiency |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-28T18%3A50%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-acs_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Well-Defined%20Poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl%20methacrylate)%20Vectors%20with%20Low%20Toxicity%20and%20High%20Gene%20Transfection%20Efficiency&rft.jtitle=Biomacromolecules&rft.au=Xu,%20F.%20J&rft.date=2010-06-14&rft.volume=11&rft.issue=6&rft.spage=1437&rft.epage=1442&rft.pages=1437-1442&rft.issn=1525-7797&rft.eissn=1526-4602&rft_id=info:doi/10.1021/bm100309y&rft_dat=%3Cacs_cross%3Ee13818600%3C/acs_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/20426406&rfr_iscdi=true |