Antiviral Activity of Electrospun Polyamide Ultrathin Fibers Against SARS-CoV‑2 Variant

The development of new strategies to produce nanomaterials that can be used as personal protective equipment with antiviral activity and low toxicity is crucial. Electrospun ultrathin fibers have attracted considerable attention due to their potential for biomedical applications, including antiviral activity. Herein, we electrospun different grades of commercially available polyamide to produce ultrathin fibers and investigate their antiviral activity against SARS-CoV-2 Gamma lineage (P.1). We evaluated the morphology, chemical composition, and mechanical properties of the ultrathin fibers. We also investigated the in vitro cytotoxicity, hemolytic activity, and antiviral activity against SARS-CoV-2 Gamma lineage (P.1) of the developed ultrathin fibers. The ultrathin fibers had the following diameters and elastic moduli: (i) unmodified crude ultrathin polyamide (PAP) 0.59 μm and 3 MPa, (ii) polyamide Biotech (PAAM) 0.74 μm and 2.2 MPa, and (iii) Amni Virus-Bac OFF polyamide (PAVB) 0.69 μm and 1.06 MPa. The ultrathin PAP fibers showed increased antiviral activity compared to the other ultrathin fibers (PAAM and PAVB). None of the electrospun fibers showed cytotoxicity at the lowest concentration (12.5%). Additionally, hemolysis tests demonstrated a nonhemolytic profile for all fiber groups, reinforcing their biocompatibility and suitability for biomedical applications. The antiviral properties of the electrospun ultrathin PAP fibers, combined with their noncytotoxic and nonhemolytic characteristics, highlight their potential to be used as personal protection against SARS-CoV-2.