Multifunctional Supramolecular Assemblies with Aggregation-Induced Emission (AIE) for Cell Line Identification, Cell Contamination Evaluation, and Cancer Cell Discrimination

It is undoubted the important role of cells in biology and medicine, but worldwide misidentified and cross-contaminated cell lines have caused much trouble in related fields. Herein, three kinds of supramolecular AIE (aggregation-induced emission) nanoassemblies were constructed by the host–guest interaction between tetraphenylethene (TPE) derivatives and cucurbit[8]­uril (CB[8]). Based on the recognized mechanism of AIE, the TPE derivatives could achieve stronger fluorescence emission and higher fluorescence quantum yield after assembling with CB[8]. Moreover, the constructed supramolecular AIE complexes obtained well-confirmed nanostructures and exhibited different sizes and shapes. Correspondingly, they generated characteristic biological properties and fluorescence enhancement of cells. Inspired by the concept of Big Data Analysis, these fluorescence signals were further transformed into a unique fingerprint of cells via linear discriminant analysis. Immediately, we realized the veracious identification between a normal cell line, two cancer cell lines, and two metastasized cancer cell lines in a qualitative analysis. More importantly, it was well used to monitor the evaluation of cross-contaminated cells and the discrimination of cancer cells. As a proper bioapplication of ideal supramolecular nanomaterials, this system was easy to learn and apply, and the whole procedure was kept to 20 min, without cell disruption, centrifugation, or washing steps.