Gene Expression and Metabolic Analyses of Nontransgenic and AtPAP 1 Transgenic Tobacco Infected with Potato Virus X (PVX)
(PVX), a species of the genus , is a plant pathogenic virus that causes severe symptoms such as mild mosaic, crinkling, necrosis, and mottling on leaves. The objectives of the present study were to investigate the effect of PVX virus infection on the metabolic system in nontransgenic and production...
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Veröffentlicht in: | Journal of agricultural and food chemistry 2022-05, Vol.70 (19), p.5838-5848 |
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Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | (PVX), a species of the genus
, is a plant pathogenic virus that causes severe symptoms such as mild mosaic, crinkling, necrosis, and mottling on leaves. The objectives of the present study were to investigate the effect of PVX virus infection on the metabolic system in nontransgenic and
production of anthocyanin pigment 1 (
1) transgenic tobacco using transcript expression analysis and metabolic profiling.
inoculation increased the gene expression of phenylpropanoid and flavonoid biosynthesis and the production of chlorogenic acid,
-coumaric acid, benzoic acid, rutin, quercetin, and kaempferol in nontransgenic tobacco leaves. However, in the
1 transgenic tobacco leaves, PVX inoculation decreased the expression of
1 and phenylpropanoid and flavonoid biosynthesis genes, and the production of phenolics and anthocyanin also declined. In contrast, the levels of amino acids and tricarboxylic acid (TCA) cycle intermediates increased after infection in the
1 transgenic plant leaves. To date, these results have not been reported previously. We suggest that PVX infection decreases
1 expression, leading to the downregulation of phenylpropanoid and flavonoid biosynthesis in transgenic plants. |
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ISSN: | 0021-8561 1520-5118 |
DOI: | 10.1021/acs.jafc.2c00974 |