Toxicity of Graphene Oxide in Nematodes with a Deficit in the Epidermal Barrier Caused by RNA Interference Knockdown of unc-52

The epidermal barrier is important for the defense of environmental organisms against the damage from engineered nanomaterials (ENMs). We employed Caenorhabditis elegans to examine the possible influence of a deficit in the epidermal barrier caused by RNA interference (RNAi) knockdown of unc-52 enco...

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Veröffentlicht in:Environmental science & technology letters 2018-11, Vol.5 (11), p.622-628
Hauptverfasser: Ding, Xuecheng, Rui, Qi, Zhao, Yunli, Shao, Huimin, Yin, Yiping, Wu, Qiuli, Wang, Dayong
Format: Artikel
Sprache:eng
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Zusammenfassung:The epidermal barrier is important for the defense of environmental organisms against the damage from engineered nanomaterials (ENMs). We employed Caenorhabditis elegans to examine the possible influence of a deficit in the epidermal barrier caused by RNA interference (RNAi) knockdown of unc-52 encoding a perlecan protein on the toxicity of graphene oxide (GO). Epidermal RNAi knockdown of unc-52 caused a functional deficit in the epidermal barrier and susceptibility to GO toxicity. Epidermal knockdown of unc-52 decreased the level of expression of fbl-1 encoding a membrane protein fibulin and sax-7 encoding a cell adhesion receptor, and epidermal knockdown of fbl-1 or sax-7 also resulted in a functional deficit in the epidermal barrier and susceptibility to GO toxicity. Additionally, epidermal knockdown of unc-52 inhibited expression of cnc-2 and prx-11 encoding two antimicrobial proteins, and epidermal knockdown of cnc-2 or prx-11 could strengthen the GO toxicity in fbl-1­(RNAi) or sax-7­(RNAi) nematodes. Our data further highlight the important function of the epidermal barrier against toxicity of environmental ENMs.
ISSN:2328-8930
2328-8930
DOI:10.1021/acs.estlett.8b00473