Preserving toxicologic activity during chromatographic fractionation of bioactive complex mixtures

Preserving toxicologic activity during chromatographic fractionation of bioactive complex mixtures

Four types of chromatographic materials were evaluated for degree of recovery of mutagenic components during column chromatography. Materials tested were silica, alumina, Florisil, and cyanopropyl-bonded silica. A combustion-generated complex mixture that was highly characterized and known to contai...

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Veröffentlicht in:Anal. Chem.; (United States) 1986-03, Vol.58 (3), p.568-572
Hauptverfasser: Lafleur, Arthur L, Braun, Andrew G, Monchamp, Peter A, Plummer, Elaine F
Format: Artikel
Sprache:eng
Schlagworte:
400102 - Chemical & Spectral Procedures
ADSORPTION
ALUMINIUM COMPOUNDS
ALUMINIUM OXIDES
Analytical chemistry
Animals
CHALCOGENIDES
Chemistry
Chromatographic methods and physical methods associated with chromatography
CHROMATOGRAPHY
Chromatography, Liquid
DATA
Drug Stability
Exact sciences and technology
EXPERIMENTAL DATA
FRACTIONATION
In Vitro Techniques
INFORMATION
INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY
LIQUID COLUMN CHROMATOGRAPHY
MATERIALS
MINERALS
Mutagenicity Tests
MUTAGENS
Mutagens - analysis
Mutagens - isolation & purification
Mutagens - toxicity
NUMERICAL DATA
Other chromatographic methods
OXIDE MINERALS
OXIDES
OXYGEN COMPOUNDS
Rats
SEPARATION PROCESSES
SILICA
SILICON COMPOUNDS
SILICON OXIDES
SORPTION
SORPTIVE PROPERTIES
SURFACE PROPERTIES
TOXIC MATERIALS
TOXICITY
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Beschreibung
Zusammenfassung:Four types of chromatographic materials were evaluated for degree of recovery of mutagenic components during column chromatography. Materials tested were silica, alumina, Florisil, and cyanopropyl-bonded silica. A combustion-generated complex mixture that was highly characterized and known to contain mutagenic components was used as the reference sample. The cyanopropyl material was found to be the most efficient material for mutagen recovery and alumina proved to be the least efficient. 15 references, 3 figures, 5 tables.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00294a017