Effect of cyanide ions (CN-) extracted from cassava (Manihotesculenta Crantz) on Alveolar Epithelial Cells (A549 cells)
[Display omitted] •Manihot esculenta (Cassava) is an important tropical tuber crop.•The leaf extract of Manihot esculenta (Cassava) contains cyanogenic glycosides, which have insecticidal properties.•CCE isolated from cassava leaves demonstrated cytotoxic effect at 400 ppm in A549 cell line. Cassava...
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Veröffentlicht in: | Toxicology (Amsterdam) 2021-12, Vol.464, p.153019, Article 153019 |
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Sprache: | eng |
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•Manihot esculenta (Cassava) is an important tropical tuber crop.•The leaf extract of Manihot esculenta (Cassava) contains cyanogenic glycosides, which have insecticidal properties.•CCE isolated from cassava leaves demonstrated cytotoxic effect at 400 ppm in A549 cell line.
Cassava (Manihotesculenta Crantz) is one of the most important root crops in tropical countries. It is a major source of cyanogenic glycosides viz. linamarin and lotaustralin, and these on breakdown liberate HCN and ketone. Cassava cyanide extract (CCE) from cassava leaves and tuber rinds were formulated as a biopesticide against certain borer insect pests of horticultural crops. Adenocarcinomic human alveolar basal epithelial cells (A549) were treated with three different concentrations (100, 200, 400 ppm) of CCE. The MTT and NRU assays showed dose-dependent cytotoxicity. The DCFH-DA assay does not show any free radical scavenging activity, whereas the NRR assay showed a reduction in the nitrile radicals with an increase in the concentration of the bioactive compound. A negative correlation was found between the concentration of the bioactive principles and mitochondrial and lysosomal functions. Various cellular assays demonstrated the cellular response of the CCE, and it was found that at higher concentration (400 ppm), the CCE exert a significant necrotic cell death rather than apoptosis. The results of the study indicated that the CCE have a remarkable tendency of anti-proliferative ability. |
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ISSN: | 0300-483X 1879-3185 |
DOI: | 10.1016/j.tox.2021.153019 |