Influence of green manure and rice straw management on soil organic carbon, enzyme activities, and rice yield in red paddy soil

•Organic C inputs significantly increased SOC compared to synthetic fertilizer.•Organic amendment quality and soil water regimes influenced SOC sequestration.•Organic amendments increased soil hydrolase activity but decreased oxidase activity.•Applying green manure resulted in higher rice yield compared to rice straw.•Organic C quality may influence the SOC composition and its agroecosystem function. Both the quantity and quality of organic amendments can influence the accumulation of soil organic carbon (SOC) and agroecosystem functioning. However, the evidences of the effect of combination of quantity and quality of organic amendments on the paddy soil quality and rice yield are scarce. An ongoing field experiment was conducted in Hunan province of China to investigate the effect of different Chinese milk vetch (Astragalus sinicus L.) and rice (Oryza sativa L.) straw management practices on the soil biochemical properties and rice yield. The experiment consisted six treatments: applying Chinese milk vetch (MV), applying early-season rice straw (S), applying early-season and late-season rice straw (DS), applying Chinese milk vetch and early-season rice straw (SMV), applying Chinese milk vetch, early-season and late-season rice straw (DSMV), and unamended organic amendment (CK). Compared with CK, SOC content was significantly increased by 13.0%, 18.5%, 11.1%, 12.3%, and 23.0% for the MV, S, DS, SMV, and DSMV treatments, respectively. No significant correlation was found between SOC content and yearly carbon inputs. Application of organic amendments increased soil hydrolase activities (β-D-cellobiosidase, β-1,4-glucosidase, β-1,4-xylosidase, β-1,4-N-acetyl-glucosaminidase and phosphatase). The geometric mean of the assayed hydrolases followed the order: S > DSMV > SMV > DS > MV > CK. Phenol oxidase was significantly higher in CK than in the applied organic amendment treatments. The lowest ratio of β-1,4-glucosidase: phenol oxidase occurred in the S treatment, suggesting that S treatment decreased microbial substrate use efficiency. The yearly average rice yield was increased by 18.6%, 8.5%, 12.3%, 14.6%, and 24.1% for MV, S, DS, SMV, and DSMV treatment with respect to CK. There was no significant correlation between SOC content and rice yield. In general, after 4-year cultivation, our results highlight the effect of quality of organic amendments on the chemical properties of SOC and its nutrient supply. Applying high quantities of diverse organic amendments