Multifunctional DNA Nanosphere Platform for Real-time Monitoring and Fluorescence Imaging-Guided Photodynamic Therapy

The DNA nanosphere (DNS) is constructed through the assembly of multifunctional Y-scaffold DNA monomers, which encompass aptamers, RNA interferences, and photodynamic therapy agents. The DNS nanoplatform facilitates efficient cellular uptake via aptamer-mediated delivery. The RNA interferences inhibit the microRNA-21 and microRNA-155, thereby enabling precise imaging guidance for photodynamic therapy, ultimately leading to apoptosis in MCF-7 cells. The platform allows for real-time monitoring of the therapeutic process, presenting a more comprehensive approach to cancer treatment. The results of in vitro and in vivo studies suggest the potential of this multifunctional DNS platform as a promising approach for cancer therapy that can be tailored to individual patients based on their specific cancer type. •The DNA nanosphere (DNS) consists of aptamers, RNA interferences, and photodynamic therapy agents.•The DNS loads various therapeutic agents to tumor cells.•The DNS selectively silence miRNAs involved in tumor development.•Real-time imaging of miRNA in cells allows for precise photodynamic therapy.