SNPgen: A portal of innovative automated tools for genotyping assay design

SNPgen is a web portal that simplifies primer design for the detection of single nucleotide polymorphisms (SNPs) and insertions/deletions (indels). It offers user-friendly tools for automating primer design, retrieve SNP details and generate primers for various genotyping methods such as ARMS-PCR and high-resolution melt (HRM) analysis. SNPgen considers factors such as GC content and melting temperature for optimal primers and allows visualization of amplicons and primers. This user-friendly portal can revolutionize genotyping workflows in various research areas. •Designs ARMS-PCR primers & mCADMA primers for SNP/indel genotyping.•User-friendly web portal for researchers (SNPgen).•Simplifies genotyping in medicine, agriculture & more.•Successful in real-world studies (PCOS, T2D, diabetic retinopathy etc.).•Portal is freely accessible via: https://snp.biotech.edu.lk.