Generation of a human embryonic stem cell (WAe001-A-47) with hVGLL4 doxycyclin-inducible expression by the PiggyBac transposon system

•A human embryonic stem cell (hESC) line (WAe001-A-47) with hVGLL4 overexpression was generated from the H1 hESC.•The hESC line (WAe001-A-47) showed the pluripotency feature similar to hESC and the normal kayotype.•The hESC line (WAe001-A-47) could be inducible-expressed hVGLL4 with doxycycline treatment. VGLL4 is a new component of the Hippo pathway and bind TEADs to compete with YAP, so as to inhibit tumor progression, but its role in stem cell and organ regeneration remains unclear. Using the PiggyBac transposon system, we generated a VGLL4 doxycycline-inducible expression human embryonic stem cell line (WAe001-A-47). The established hESC line retains its normal morphology and pluripotency markers with in vitro differentiation potential, as well as a normal karyotype.