Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs

Generation of a human iPSC line GIBHi002-A-2 with a dual-reporter for NKX2-5 using TALENs

The human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; Pur...

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Veröffentlicht in:Stem cell research 2021-01, Vol.50, p.102120-102120, Article 102120
Hauptverfasser: Zhou, Min, Wei, Rui, Jiang, Yu, Fu, Jian, Liu, Yuqing, Yang, Bo, Yu, Baorong, Lin, Yun, Ran, Xinru, Lai, Wing-Hon, Chu, Meng, Hu, Yang, Yang, Jiayin, Tse, Hung-Fat
Format: Artikel
Sprache:eng
Schlagworte:
Biotechnology & Applied Microbiology
Cell & Tissue Engineering
Cell Biology
Life Sciences & Biomedicine
Science & Technology
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Zusammenfassung:The human transcription factor NKX2-5 plays an important role in cardiac formation and development, and thus it can be used for isolation of cardiomyocytes (CMs) differentiated from human pluripotent stem cells (hPSCs). Here, we knocked-in enhanced GFP (eGFP) and Pac (a puromycin resistant gene; PuroR) into the exon 1 coding region of NKX2-5 from a human iPSC line iPSN0003 using TALENs. The generated GIBHi002-A-2 enables us to monitor and optimize cardiac differentiation procedures via the cardiac progenitor cells (CPCs), as well as to isolate iPSC-derived CMs for drug screening.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2020.102120