Stemphylium lycopersici immobilized in mesoporous of type MCM 48 as biocatalyst for ω-transamination reactions

The ability of Stemphylium lycopersici immobilized in MCM 48 zeolite structure as biocatalyst for ω-transamination reactions was evaluated. The conversion capacity for rac-1-methylbenzylamine, and also rac-1,2,3,4-tetrahydro-1-naphthylamine was verified in order to ascertain the potential of this biocatalyst in relation to the bioconversion of amines with different side chains when using immobilized crude enzyme extract in zeolite. In this first reaction (after 24 h), a conversion of 23% to acetophenone and 27% to tetralone was obtained. Observing the promising values obtained in the kinetic resolution reactions, an experimental design was carried considering the amount (mg) out of biocatalyst present in the support and the amount of ionic liquid used Central Compound Rotational Design (CCRD2 complete). After optimization, values close to 36% conversion were achieved. In the present work, there were performed 25 cycles of 18 h with Zeolite MCM48 with the addition of ionic liquids (LI). The presence of LI's helps with stability and still acts as structure drivers, which may have helped by providing a large number of recycles. In this analysis, 87% of the initial activity was maintained. [Display omitted] •Stemphylium lycopersici immobilized in MCM 48 zeolite structure showed potential as a biocatalyst for ω-transamination reactions.•Conversion capacities for different amines were evaluated, with promising results obtained.•An experimental design was carried out to optimize the reaction conditions, achieving close to 36% conversion.•The addition of ionic liquids (LI) helped maintain stability and allowed for a high number of recycles.•Overall, 87% of the initial activity of the biocatalyst was maintained throughout the analysis.