A comparative novel green reversed-phase high-performance liquid chromatography method for simultaneous determination of ivabradine and carvedilol in tablets and human plasma using UV and fluorescence detectors

A rapid, green, and sensitive dual detection technique method was applied using both UV and fluorimetric detection to determine ivabradine and carvedilol in their pure form, combined tablet formulation, and in the spiked human plasma. The method was achieved by using HyperClone™ MOS C8 column (150 × 4.6 mm, with particle size 5 μm), and a mixture composed of (70:10:20%v/v) ethanol: methanol: 0.02 M potassium phosphate buffer adjusted to pH = 3 b y using o-phosphoric acid. The dual detection technique was performed at 210 nm in the case of UV detection, and at 337 nm after excitation at 282 nm in the case of fluorimetric detection with a flow rate of 1 mL/min. The retention time in the proposed method was found to be 2.13 and 4.72 min for carvedilol and ivabradine respectively (UV detection) and 2.16 and 4.5 min for carvedilol and ivabradine respectively (fluorescence detection). It was found to be a rectilinear method (r > 0.999), precise (RSD