Cyanotoxins associated with macrophytes in Berlin (Germany) water bodies – Occurrence and risk assessment

Fatal dog poisoning after uptake of neurotoxic cyanobacteria associated with aquatic macrophytes in Tegeler See (Berlin, Germany) raised concerns about critical exposure of humans, especially children, to cyanotoxins produced by macrophyte associated cyanobacteria during recreational activity. From 2017 to 2021 a total of 398 samples of macrophytes washed ashore at bathing sites located at 19 Berlin lakes were analysed for anatoxins, microcystins, and cylindrospermopsins, as were 463 water samples taken in direct proximity to macrophyte accumulations. Cyanotoxins were detected in 66 % of macrophyte samples and 50 % of water samples, with anatoxins being the most frequently detected toxin group in macrophyte samples (58 %) and cylindrospermopsins in water samples (41 %). Microcoleus sp. associated with the water moss Fontinalis antipyretica was identified as anatoxin producing cyanobacterium in isolated strains as well as in field samples from Tegeler See. Anatoxin contents in macrophyte samples rarely exceeded 1 μg/g macrophyte fresh weight and peaked at 9. 2 μg/g f.w. Based on established toxicological points of departure, a critical anatoxin content of macrophyte samples of 3 μg/g f.w. is proposed. Five samples, all taken in Tegeler See and all associated with the water moss Fontinalis antipyretica, exceeded this value. Contents and concentrations of microcystins and cylindrospermopsins did not reach critical levels. The potential exposure risks to anatoxins for children and dogs are assessed and recommendations are given. [Display omitted] •From 2017 to 2021, a total of 398 macrophyte samples were analysed for associated cyanobacteria and cyanobacterial toxins.•Anatoxin-producing Microcoleus sp. (cyanobacteria) is often associated with the water moss Fontinalis antipyretica.•Anatoxin contents of macrophyte samples are highly variable in space and time.•Hazard assessment suggests that toxigenic cyanobacteria/macrophyte assemblages are a relatively minor risks for humans.