Variation of airborne DNA mass ratio and fungal diversity in fine particles with day-night difference during an entire winter haze evolution process of Central China

Airborne fungi are a primary component of bioaerosols and proved to impact human health and climatic change. Deoxyribonucleic acid (DNA) is the essential component of most living organisms with relatively stable physicochemical properties. Little is known about day-night and pollution-episode differences of DNA mass ratio and fungal community in fine particles (PM2.5) during serious winter haze events in China. Here we collected twenty-nine PM2.5 samples every day and night during an entire winter haze evolution process in a megacity of Central China, Wuhan. DNA extraction and high-throughput sequencing methods were adopted to analyze fungal community. Results showed that mass ratio of DNA in PM2.5 (RD/P %) changed with pollution process and showed significant negative correlations with PM2.5 concentration (r = −0.72, P