Phenolics pattern of cut H3O rose flowers during floral development

•The content of secondary metabolites were changed during flower development of cut H3O rose flowers.•Dominant compounds during the different days of flower development were modified.•Cyanidin and Pelargonidin were two anthocyanins were detected in methanolic extract of cut H3O rose flowers. An experiment was conducted to evaluate the phenolic acids and flavonoids contents from flower bud to flower senescence stages (7 stages) in cut H3O roses every other day. Some phenolic acids (Gallic, Caffeic, Caftaric, Chlorogenic, Chicoric, Coumaric, Sinapic, and Ferulic) and flavonoids (Catechin, Pelargonidin chloride, Cyanidin, Kaempferol, and Quercetin) were detected in the methanolic extract of rose petals by HPLC. Data analysis revealed that Gallic acid content did not significantly (P ≤ 0.05) affects from the flower bud to the senescence stages. However, it’s found that Caftaric acid, Chlorogenic acid, Coumaric acid, Ferulic acid, Chicoric acid, Quercetin, and Kaempferol (at P ≤ 0.05), Catechin, Caffeic acid, Sinapic acid, Cyanidin, and Pelargonidin chloride contents (at P ≤ 0.01) significantly affected during flower opening stages. The highest contents of phenolics during development of cut H3O rose flowers were as follow: day 1 [Coumaric acid], day 3 [Caftaric acid, Ferulic acid, and Kaempferol], day 5 [no compound was found], day 7 [Sinapic acid and Cyanidin], day 9 [Chlorogenic acid], day 11 [Chicoric acid and Quercetin], and day 13 [Caffeic acid, Catechin, and Pelargonidin chloride]. The results showed that flower development stages can be suggested to consider the phenolic compounds required to use in the therapeutic process.