A long-wavelength mitochondria-targeted fluorescent probe for imaging of peroxynitrite during dexamethasone treatment

[Display omitted] •Probe is capable of detecting ONOO− with a longwave fluorescence emission.•TL could monitor high concentration of dexamethasone-induced an up-regulation of ONOO−.•Probe has mitochondrial targeting capability.•Probe have good selectivity and low detection limit.•The imaging demonst...

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Veröffentlicht in:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2023-05, Vol.292, p.122429, Article 122429
Hauptverfasser: Tang, Jun, Li, Ziyi, Qiang, Chuchu, Han, Yan, Yang, Lifang, Zhu, Li, Dang, Tan, Chen, Gairong, Ye, Yong
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Sprache:eng
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Zusammenfassung:[Display omitted] •Probe is capable of detecting ONOO− with a longwave fluorescence emission.•TL could monitor high concentration of dexamethasone-induced an up-regulation of ONOO−.•Probe has mitochondrial targeting capability.•Probe have good selectivity and low detection limit.•The imaging demonstrated its value of practical application. Peroxynitrite (ONOO−), as a strong oxidizing reactive nitrogen substance (RNS), is generated endogenously by cells. Its visualization research is crucial to understand relevant disease processes. Herein, we reported a long-wavelength mitochondria-targeted fluorescence “turn on” probe TL. The probe TL could react with ONOO− by using 4-(Bromomethyl)benzeneboronic as a reactive site, which exhibited outstanding characteristics for detection of ONOO−, thus improving response time (about 50 s), sensitivity (DL, 10.1 nM), and emission wavelength (667 nm). Besides, TL displayed well mitochondria targeting and biological visualizing of exogenous and endogenous ONOO− in biological systems. Finally, TL was used to monitor high concentration of dexamethasone-induced an up-regulation of ONOO−. This indicated that TL has excellent potential to study the fluctuation of ONOO− in the physiological and pathological system.
ISSN:1386-1425
1873-3557
DOI:10.1016/j.saa.2023.122429