A long-wavelength mitochondria-targeted fluorescent probe for imaging of peroxynitrite during dexamethasone treatment
[Display omitted] •Probe is capable of detecting ONOO− with a longwave fluorescence emission.•TL could monitor high concentration of dexamethasone-induced an up-regulation of ONOO−.•Probe has mitochondrial targeting capability.•Probe have good selectivity and low detection limit.•The imaging demonst...
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Veröffentlicht in: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2023-05, Vol.292, p.122429, Article 122429 |
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Sprache: | eng |
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Zusammenfassung: | [Display omitted]
•Probe is capable of detecting ONOO− with a longwave fluorescence emission.•TL could monitor high concentration of dexamethasone-induced an up-regulation of ONOO−.•Probe has mitochondrial targeting capability.•Probe have good selectivity and low detection limit.•The imaging demonstrated its value of practical application.
Peroxynitrite (ONOO−), as a strong oxidizing reactive nitrogen substance (RNS), is generated endogenously by cells. Its visualization research is crucial to understand relevant disease processes. Herein, we reported a long-wavelength mitochondria-targeted fluorescence “turn on” probe TL. The probe TL could react with ONOO− by using 4-(Bromomethyl)benzeneboronic as a reactive site, which exhibited outstanding characteristics for detection of ONOO−, thus improving response time (about 50 s), sensitivity (DL, 10.1 nM), and emission wavelength (667 nm). Besides, TL displayed well mitochondria targeting and biological visualizing of exogenous and endogenous ONOO− in biological systems. Finally, TL was used to monitor high concentration of dexamethasone-induced an up-regulation of ONOO−. This indicated that TL has excellent potential to study the fluctuation of ONOO− in the physiological and pathological system. |
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ISSN: | 1386-1425 1873-3557 |
DOI: | 10.1016/j.saa.2023.122429 |