Surface enhanced fluorescence immuno-biosensor based on gold nanorods

[Display omitted] •Development of biosensors based on Au nanorods and surface-enhanced fluorescence.•Biosensing based on sandwich immunoassays for monitoring the molecular recognition.•Highly positive response for anti-BSA detection using antigen–antibody interaction.•Conversion of spectroscopic data into a digital protocol, simplifying data analysis.•Contributing for advancing SEF and AuNRs as an efficient biosensors platforms. Gold nanoparticles (AuNPs) are attractive structures for biosensing, most due to different properties at nanoscale and biocompatibility. Localized surface plasmon resonance (LSPR) is one of these properties; LSPR enable the electromagnetic field enhancement closer to metallic surface, which allows surface-enhanced spectroscopies, like surface enhanced fluorescence (SEF). In this study, an immuno-biosensor based on gold nanorods (AuNRs) and SEF was constructed for simple and fast analysis to detect albumin antibody (anti-BSA) using antigen–antibody (anti-BSA/BSA) interaction as the biorecognition model. AuNRs were presented in two distinct configurations, in suspension (S-AuNRs) and adsorbed on glass slides (AuNRs-chip), and the detection was performed through an extrinsic method, wherein the SEF signal of a reporter molecule (IR-820 cyanine-type dye) was monitored. The analyte detection was evidenced by SEF mapping, where the average signal in the presence of anti-BSA was three times more intense than for the assay in the absence of analyte. A digital protocol was proposed to simplify the spectroscopic data analysis and reduce the intensity variability; in this protocol the number of positive events in the presence of anti-BSA is much larger (around two times) compared to the absence of analyte. The AuNRs based SEF immuno-biosensor allowed an efficient and simple analysis with specific biorecognition and may contribute as an efficient spectroscopy platform for immuno-biosensing.