A new chromone functionalized isoqunoline derived chemosensor with fluorogenic switching effect for selective detection of Zn2+ in real water samples and living cells

A novel chromone-isoquinoline derived Schiff base ENO was rationally developed as a chromogenic and fluorogenic “turn-on” chemosensor for Zn2+ detection. ENO presented here demonstrated outstanding capability in monitoring Zn2+ ions in real water samples, living cells as well as the on-site assay kit. [Display omitted] •A novel chromone functionalized isoquinoline derived chemosensor ENO was developed for selective Zn2+ detection.•ENO exhibited good sensing response toward Zn2+ with a large red shift of 130 nm.•Limit of detection (LOD) of ENO for Zn2+ was as low as 34 nM.•ENO was utilized as a powerful tool for monitoring Zn2+ in real samples.•ENO-prepared test strips were employed for quantitative detection of Zn2+ ions through a colorimetric manner. In this work, a selective chemosensor, (E)-N′-((4-oxo-4H-chromen-3-yl)methylene)isoquinoline-1-carbohydrazide (ENO), was rationally developed for colorimetric and fluorogenic detection of Zn2+ ions. It was readily synthesized from 4-oxo-4H-chromene-3-carbaldehyde and isoquinoline-1-carbohydrazide via one-step Schiff reaction. ENO exhibited excellent fluorescent response performances toward Zn2+ over a wide pH range in EtOH/H2O media, including a distinguished color change from colorless to gold, a low limit of detection (LOD) value (34 nM), strong complexation ability (1.36 × 105 M−1) and rapid identification (2 min). The sensing mechanism of ENO toward Zn2+ was proposed on the basis of the chelation-enhanced fluorescence (CHEF) process, which was further supported by IR studies and the density functional theory (DFT) calculation. Moreover, ENO presented here demonstrated outstanding capability in monitoring trace level of Zn2+ ions in real water samples, living cells as well as the on-site assay kit.