Quick and sensitive colorimetric detection of amino acid with functionalized-silver/copper nanoparticles in the presence of cross linker, and bacteria detection by using DNA-template nanoparticles as peroxidase activity

[Display omitted] •Ag/Cu functionalized with poly citric acid was synthesized by chemical method.•The probe was used to detection of cysteine in Mg2+ presence.•The DNA template was used to Ag/Cu/PCA for detection of S. pneumoniae.•The cysteine and S. pneumoniae detection were conducted from real samples with the fabricated probe. In this project, poly (citric acid) (PCA) functionalized on nano Ag/Cu was synthesized by chemical analysis method. The nano probe was applied to detection of cysteine by using the magnesium (II) ions as a cross linker. The characterization of Ag/Cu/PCA nano probe was studied by using the UV–visible, morphological microscopy, dynamic light scattering, and zeta potential analyzer. The zeta potential and size of Ag/Cu/PCA were −38.0 mV and 18.0 nm, respectively. The prepared nano probe shows rapid response for detection of cysteine. The detection limit of Ag/Cu/PCA nano probe was 0.07 nM. Additional, the Ag/Cu/PCA nanoparticles was applied to cysteine detection from real samples in the presence of amino acids compounds. Rapidly and sensitive determination of Streptococcus pneumoniae is substantial for food safety and human health. The DNA-Ag/Cu/PCA were prepared as a template in chemical method and experimented as a bio-receptor for the cell bacteria detection as peroxidase-like catalytic process. The DNA-Ag/Cu/PCA nano probe shows a linear dynamic concertation range of Streptococcus pneumoniae via detection limit about 65 CFU/mL. The project presents that the DNA-Ag/Cu/PCA could detect the biological and bacterial samples via high accuracy.