A ratiometric and colorimetric fluorescent probe designed based on FRET for detecting SO32−/HSO3− in living cells and mice

A ratiometric and colorimetric fluorescent probe designed based on FRET for detecting SO32−/HSO3− in living cells and mice

[Display omitted] •Structure NBC was firstly used as a fluorescent donor for FRET probes.•TFBN owns 239 nm Stokes shift and 140 nm emission band gap.•The probe TFBN can quantitatively detect sulfite in the bilinear range.•TFBN has been applied to biological imaging in living cells and mice. Based on...

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Veröffentlicht in:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2021-12, Vol.263, p.120183, Article 120183
Hauptverfasser: Shen, Weiliang, Xu, Hanhan, Feng, Jing, Sun, Wei, Hu, Guoxing, Hu, Yonghong, Yang, Wenge
Format: Artikel
Sprache:eng
Schlagworte:
Fluorescent probe
FRET
Ratiometric and colorimetric
SO2 derivatives (SO32−/HSO3−)
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Zusammenfassung:[Display omitted] •Structure NBC was firstly used as a fluorescent donor for FRET probes.•TFBN owns 239 nm Stokes shift and 140 nm emission band gap.•The probe TFBN can quantitatively detect sulfite in the bilinear range.•TFBN has been applied to biological imaging in living cells and mice. Based on the principle of FRET, we have developed a ratiometric and colorimetric fluorescent probe TFBN, which can specifically recognize SO2 derivatives (SO32−/HSO3−), and exhibit a transition from red to green fluorescence under 405 nm excitation. The probe TFBN owns the advantages of short response time (
ISSN:1386-1425
DOI:10.1016/j.saa.2021.120183