Green spectrophotometric methods for the determination of a binary mixture of lidocaine hydrochloride and cetylpyridinium chloride in the presence of dimethylaniline

Three green, simple, precise, accurate and sensitive spectrophotometric methods were developed for the determination of a binary mixture of lidocaine hydrochloride (LDC) and cetylpyridinium chloride (CPC) in the presence of dimethylaniline (DMA). In the three methods, the interference of DMA spectrum is eliminated using the ratio subtraction method. Method (A) depended on determining LDC and CPC by measuring the first derivative of the ratio spectra (1DD) at 271.0 and 268.4 nm, respectively. Method (B) was the ratio difference (RD), based on dividing the absorption spectrum of the binary mixture by a standard spectrum of CPC or LDC, then measuring the amplitude difference of the ratio spectra (∆P) between 231.2 and 240.0 nm for LDC and between 242.8 and 258.0 nm for CPC. Method (C) based on the application of dual wavelength coupled with the isoabsorptive point method. This was achieved by measuring the absorbance difference (∆A) between 243.0 and 268.6 nm for the determination of LDC, followed by application of isoabsorptive point method comprised of measurement the total content of the mixture of LDC and CPC at their isoabsorptive point at 240.0 nm. The content of CPC was obtained by subtraction. The specificity of the developed methods was investigated by analyzing laboratory prepared mixtures containing different ratios of LDC and CPC in presence of DMA. The proposed methods displayed useful analytical characteristics for the determination of LDC and CPC in bulk powder and their combined dosage form. The obtained results were statistically compared with those obtained by the official methods, showing no significant difference with respect to accuracy and precision. [Display omitted] •Three simple and eco-friendly spectrophotometric methods were successfully applied.•Analysis of a binary mixture in the presence of an impurity with overlapped spectra.•Validated according to the ICH guidelines & compared to the official methods.•The methods were applied for the analysis of dosage form without prior separation.•Easily implemented in QC labs lacking liquid chromatographic instruments.