Etching-controlled suppression of fluorescence resonance energy transfer between nitrogen-doped carbon dots and Ag nanoprisms for glucose assay and diabetes diagnosis
Numerous methods have been developed for glucose detection, only few cases can be really applied in clinical diagnosis. Herein, we report a new approach to achieve the detection of glucose in clinical samples and distinguishing the diabetic patients with healthy ones. Specifically, a fluorescence re...
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Veröffentlicht in: | Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2020-12, Vol.242, p.118713, Article 118713 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Numerous methods have been developed for glucose detection, only few cases can be really applied in clinical diagnosis. Herein, we report a new approach to achieve the detection of glucose in clinical samples and distinguishing the diabetic patients with healthy ones. Specifically, a fluorescence resonance energy transfer (FRET) system is established first, where nitrogen-doped carbon dots (N-CDs) and Ag nanoprisms (AgNPRs) with good spectral overlap act as energy donor and acceptor, respectively. Then, the FRET can be inhibited through oxidative etching of the energy acceptor in the presence of glucose and glucose oxidase, where hydrogen peroxide is generated to transform AgNPRs into Ag+ ions. Based on the turn-on fluorescent signal versus glucose concentration, a new method for quantitative detection of glucose is developed. This etching-induced analytical method is simple, reliable, robust and cost-effective, which is promising to assist the doctors to clinically diagnose diabetes and other diseases related to metabolic disorders.
A turn-on fluorescent sensing platform for clinical glucose detection and diabetes diagnosis is developed based on controlled etching of silver nanoprisms. [Display omitted]
•A turn-on fluorescent method is developed for glucose detection based on the etching-controlled suppression of FRET.•Fluorescent carbon dots and Ag nanoprisms are employed as energy donor and acceptor of FRET, respectively.•FRET can be restrained through transforming Ag nanoprisms into Ag+ by H2O2 generated from catalytic oxidation of glucose.•This method has been applied for glucose detection in clinical samples and distinguishing diabetics from healthy persons. |
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ISSN: | 1386-1425 1873-3557 |
DOI: | 10.1016/j.saa.2020.118713 |