RNA-responsive fluorescent carbon dots for fast and wash-free nucleolus imaging

RNA as a carrier of genetic information plays a critical role in various physiological processes. RNA-rich nucleolus is usually employed as an important biomarker for many malignant diseases. Herein, RNA-responsive fluorescent carbon dots (CDs) were synthesized by a simple microwave method. Due to the presence of cationic benzothiazolium groups in the CDs, a “turn-on” fluorescence signal was achieved between CDs and RNA. The CDs exhibit excellent RNA selectivity and a good linear relationship with a detection limit of 0.62 μg/mL. The small particle size, polarity sensitivity and RNA response behavior of CDs realized fast and wash-free nucleolus imaging effectively. Overall, these CDs provide a powerful potential tool for monitoring cell nucleus activity and elucidating RNA dynamics. [Display omitted] •CDs are responded to RNA sensitively and selectively with more than 15-fold fluorescence enhancement.•CDs have intrinsic nucleolus targeting ability without any post modification.•CDs can penetrate cell membrane and nucleus membrane within 2 min and achieve wash-free nucleolus imaging effectively.