Insights into prokaryotic metataxonomics and predictive metabolic function in field-scale anaerobic digesters treating various organic wastes

The operational temperature and nature of the substrates are two critical parameters in shaping the functional microbiota and determining the biogas productivity of field-scale anaerobic digesters. Elucidating microbial ecological configurations and dynamics as a function of parametric (temperature, pH, substrate variability etc.) variations is pivotal for effective process management, improving the process stability and digestion efficiency of large field-scale anaerobic digesters. Seventeen field-scale digesters treating food waste leachate, municipal wastewater sludge (MWS), and manure at 10 different sites across the Republic of Korea were studied to assess how substrate variations, including co-digestion and seasonal variation, affect biogas productivity and the compositional dynamics of the digester microbiota. The 16S rRNA gene metataxonomics and predictive metabolic pathway profiling revealed significant differences in the phylogenetic composition of the microbiota between the digester types depending on the presence of MWS in the substrate (p