A proteomic analysis of Wickerhamomyces anomalus incubated with chitosan reveals dynamic changes in protein expression and metabolic pathways

In our previous studies we determined that, chitosan can significantly increase the biological control efficiency of the antagonistic yeast Wickerhamomyces anomalus against Penicillium expansum in table grapes. However, the molecular mechanism of chitosan enhancing the yeast's disease resistance has not been explored. In this study, we investigated the role of chitosan in stress tolerances and molecular changes of W. anomalus using TMT proteomics techniques. The results showed that after 1% (w v-1) chitosan culture, the oxidative stress tolerance of W. anomalus was increased. In addition, the expression of proteins related to growth, energy metabolism, membrane function, cell regulation and stress response of W. anomalus was up-regulated. Generally, incubation of the yeast with chitosan can significantly up-regulate proteins and metabolic pathways which are important for the yeast survival, adaptation, and reproduction. The results of this study can provide a theoretical basis for the combination of chitosan as an exciter and to improve the antagonistic efficacy of W. anomalus. •Chitosan increased oxidative stress tolerance of W. anomalus.•Proteins related to growth and stress response of W. anomalus were up-regulated.•Pathways of the yeast survival and reproduction were enhanced.•The yeast membrane function and cellular regulation were promoted.