Proteomics for Brassinosteroid signalling: Understanding Brassinosteroids mediated stress responses through advanced proteomics

Brassinosteroids (BRs), class of steroidal phytohormones have a crucial role in various physiological mechanisms and involved in plant defense against different biotic and abiotic stresses. Understanding BRs action mechanism is very critical to promote plant development and boosting agricultural productivity. The mechanism of BRs action and signalling pathway is very intricate which requires exploration through various tools and techniques. Phytoproteomics had revealed the many cellular modifications induced via BRs signalling and identified potential functional metabolites involved in plant adaptation to stress conditions under BRs application. Additionally, proteomics provided the insights of activation events of receptor kinases and downregulation of various components via phosphorylation which cannot be analyzed by the genomics tools. So, the knowledge gap about the BRs signalling can be filled through various advanced proteomics tools that increase the applications of BRs in agricultural practices. This review summarizes the recent developments in knowledge of BRs signalling mechanism using various phytoproteomics tools. [Display omitted] •Recent developments in understanding Brassinosteroids mechanism and stress signalling.•Advanced proteomics tools to decipher the insights of stress signalling in plants.•Proteomics tools application in solving the complex mechanism of Brassinosteroid action.