L-histidine makes Ni2+ ‘visible’ for plant signalling systems: Shading the light on Ni2+-induced Ca2+ and redox signalling in plants

Nickel is both an important nutrient and an ecotoxicant for plants. Organic ligands, such as L-histidine (His), play a key role in Ni2+ detoxification. Here, we show that His (added together with 0.01-10 mM Ni2+) decreases Ni2+ toxicity to Arabidopsis thaliana roots not only as a result of a decreas...

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Veröffentlicht in:Plant physiology and biochemistry 2024-10, p.109227, Article 109227
Hauptverfasser: Mackievic, Viera, Li, Yalin, Hryvusevich, Palina, Svistunenko, Dimitri, Seregin, Ilya, Kozhevnikova, Anna, Kartashov, Alexander, Shabala, Sergey, Samokhina, Veranika, Rusakovich, Alina, Cuin, Tracey A., Sokolik, Anatoliy, Li, Xuewen, Huang, Xin, Yu, Min, Demidchik, Vadim
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Sprache:eng
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Zusammenfassung:Nickel is both an important nutrient and an ecotoxicant for plants. Organic ligands, such as L-histidine (His), play a key role in Ni2+ detoxification. Here, we show that His (added together with 0.01-10 mM Ni2+) decreases Ni2+ toxicity to Arabidopsis thaliana roots not only as a result of a decrease in Ni2+ activity, but also via the induction of signalling phenomena important for adaptation such as the generation of reactive oxygen species (ROS) and cytosolic Ca2+ transients. With the use of EPR spectroscopy, we demonstrate that Ni-His complexes generate hydroxyl radicals that is not detected by the addition of Ni2+ or His separately. Similarly, Ni-His complexes, but not Ni2+, activate Ca2+ influx and K+ efflux currents in patch-clamped root protoplasts resulting in distinct cytosolic Ca2+ signals and a transient K+ release. His prevented programmed cell death symptoms (cytoplasm shrinkage, protease and endonuclease activation) induced by Ni2+ and inhibited Ni2+ accumulation at [Ni2+]>0.3 mM. Intriguingly, priming of roots with Ni-His stimulated plant resistance to Ni2+. Overall, these data show that His triggers ROS-Ca2+-mediated reactions making Ni2+ ‘visible’ for plant signalling machinery and facilitating adaptation to the excess Ni2+. [Display omitted] •In Arabidopsis thaliana, histidine decreases Ni2+ inhibitory effects on growth.•Redox-active Ni-His complexes induce ROS-Ca2+ signalling events in plant cells.•Priming of Arabidopsis with Ni-His complexes stimulates resistance to Ni2+.
ISSN:0981-9428
DOI:10.1016/j.plaphy.2024.109227