Fabrication of electrospun polycaprolactone based membrane coated with curcumin chitosan nanoparticles and gelatin for wound dressing application

Besides the escalation in chronic wound diseases, the issue of proper wound care has become more necessary. The objective of this study is to design and fabricate a wound care membrane for a remarkable wound-healing treatment by retaining moisture and minimizing damage during wound dressing changes. Utilizing the electrospinning technique, a dual-layer membrane was prepared, comprising a poly (ε-caprolactone) and poloxamer 407 (PCL/POX) polymers coating with a curcumin loaded ortho-carboxymethyl chitosan nanoparticle (CurNP) and gelatin. Scanning electron microscope and Fourier transform infrared spectroscopy measurements confirmed the successful coating of CurNP and gelatin on PCL/POX membrane (denoted as CurNP-G). CurNP-G membranes showed a sustained curcumin release over 48 h, and the membrane extracts exhibited extremely low toxicity against normal fibroblast cells. In the excisional wound mouse, CurNP-G membrane treatments showed a significantly higher wound closure and tissue regeneration compared to the control PCL/POX membrane. Moreover, expression of wound healing-related genes such as Kdr, Hif1a, and Col3a1 decreased, demonstrating the therapeutic effect of CurNP-G membranes on the healing process. The obtained results in this study show the highly therapeutic potential of CurNP-G membranes for skin wound healing application. [Display omitted]