Bio electrochemical and in-vitro bioactivity evaluation of Araucaria columnaris interceded α-MoO3 nano rods fused chitosan-sodium alginate scaffold for tissue engineering applications

•α-MoO3 with highly crystalline nature and nanorod morphology were effectively synthesized using Araucaria columnaris leaf extract.•Chitosan-sodium alginate and MoO3 nanorods were combined to form biomimetic scaffolds.•MoO3 nanorods exhibits strong antibacterial activity on the specific pathogens (S. aureus and E. coli).•Potentiality for targeting breast cancer cells confirmed by cytotoxicity against MCF-7 cell lines.•Superior implant bioactivity and tissue regeneration budding was revealed by bio electrochemical studies. Araucaria columnaris leaf extract-mediated molybdenum trioxide (MoO3) nanorods were synthesized using ammonium molybdate as a precursor. Biomimetic scaffolds were prepared by solvent casting using chitosan-sodium alginate and MoO3 nanorods. Physico-chemical characteristics of MoO3 nanorods and biomimetic scaffolds were scrutinized using appropriate characterization techniques. The XRD and FE-SEM analysis claimed that molybdenum has an orthorhombic structure with the morphology of α-phase and the nanorods, respectively. TGA analysis on MoO3 nanorods retained 86.4 wt.% of mass when its temperature extended to 935 °C. The antioxidant and antibacterial properties of MoO3 nanorods and their biomimetic scaffolds have been examined. In vitro, the bioactivity of MoO3 nanorods and their biomimetic scaffolds was assessed using SBF for biomineralization and PBS for swelling and protein absorption. The MoO3 nanorods and scaffolds show better in vitro cytotoxicity against adenocarcinoma- MCF-7 cell lines at 82 % and 75 %, respectively. Bio-electrochemical behaviours of a model Ti alloy with nanocomposite scaffold against PBS solution were investigated, and its biomimetic scaffold with concertation of α-MoO3 500 mg offered a better tissue regeneration response. [Display omitted]