Synthesis of CdTe quantum dots using Glutathione promoted in Vitro Interaction between Lysozyme and Flavanoids: An investigation through spectroscopic methods

•First report on interaction of lysozyme and flavonoids in CdTe quantum dots.•Quenching parameters confirmed the mode of interaction between flavonoids and lysozyme.•Elucidate the size dependent quantum dots behavior on Lyso-Flavonoids system.•It serve as a foundation for the use of CdTe QDs in medication administration.•This study further develop the application of QDs for clinical research. We investigated the interaction of four flavonoids in this study, such as 4′,5,7-trihydroxy-isofavonethe or 5,7-dihydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one (Genistein-GES), 5,7-Dihydroxy-2-phenyl-4H-1-benzopyran-4-one (Chrysin-CRS), 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxychromen-4-one (Quercetin-QRS) and 7-Hydroxy-3-(4-hydroxyphenyl)-4H-1-benzopyran-4-one (Daidzein-DIA), [0–5 × 10−6 M] and lysozyme without and the existence of the GSHCdTe quantum dots. Here, the spectrofluorimetric technique is used to investigate the interaction and quenching observations are rationalized in terms of a static quenching way. The binding constant (K) and the number of binding sites (n) are calculated based on the fluorescence quenching data. The affinities of flavonoids and lysozyme system enhance with smaller size quantum dots. The as-prepared GSH-capped QDs clearly reveal the linkage attractions of lysozyme and flavonoids enhanced with diminishing the quantum dots size and the interaction depends on physicochemical and environmental factors. [Display omitted]