Five secondary metabolites from mangrove endophytic fungus Fusarium. proliferatum NSD-1

•Five new polyketides isolated from mangrove endophytic fungus Fusarium proliferatum•Solved absolute configuration of polyketide 2 by X-ray single-crystal diffraction•Usage of 13C NMR calculation and DP4+ probability analysis in structure elucidation•Polyketide 4 exhibited potential IL-1β inhibitory activity Five undescribed polyketides, including two talaketide derivatives (1, 4), two asperpentenone derivatives (2, 3), one phomaligol derivative (5) together with one known compound (6) were obtained from mangrove endophytic fungus Fusarium proliferatum NSD-1. The constitution of compounds 1–5 were determined by HRESIMS, 1D and 2D NMR spectra. Their absolute configurations were established by single-crystal diffraction, calculated electronic circular dichroism spectra, hydrolysis reaction, 13C NMR calculation and DP4+ probability analysis. In bioassays, compounds 1 and 5 showed moderate cytotoxicity against cancer cell lines SW480 and A549. Moreover, compound 4 showed potential IL-1β inhibitory activity. [Display omitted]