A cytostatic drug from the class of triazine derivatives: Its properties in aqueous solutions, cytotoxicity, and therapeutic activity

•Substance (a) is compatible with water and demonstrates high stability in time in neutral and alkaline medium.•Substance (a) affinity is comparable for the aqueous and lipid phases.•Substance (a) possesses high cytostatic effect towards Capan-2 and SK-MEL-1 cell lines.•Substance (a) showed high therapeutic activity in model of abdominal carcinomatosis. We present a comprehensive study of a water-soluble substance from the class of triazine derivatives alkylating agents. The performed physicochemical investigations of this cytostatic drug’s aqueous solutions include the measurements of density, viscosity, speed of sound, refraction index, stability, solubility, distribution between water and octan-1-ol, as well as electronic structure calculations and molecular dynamics modelling. Biologically, the substance was investigated for its cytotoxicity as well as in vitro (on Capan-2 and SK-MEL-1 cell lines) and in vivo (transplantable Ehrlich ascites carcinoma model) therapeutic activity.