Interaction of doxorubicin hydrochloride in the presence of, mixed aggregate of ibuprofen sodium and cationic lipid

This research was highlighted the importance of mixed aggregates based on drug-lipid to improve the interaction of one or more drugs in aqueous medium. This mixed aggregate can significantly inhibit the growth of MCF-7 cells, and has great potential for anti-inflammatory and cancer treatment after tumor removal. The cationic lipid (CL) has been synthesized with multistep reaction and is characterized by 1H NMR and mass spectroscopy. CL and ibuprofen (IBF) physiochemical analysis was investigated using surface tension, conductance, dynamic light scattering (DLS), and electron microscope transmission (TEM). The strong synergism between mixed aggregate (LIB) system CL and IBF was observed. The study on UV spectroscopy measured that the LIB mixed aggregate showed optimum binding to doxorubicin hydrochloride (DOX) and the binding constant Log Kb was 6.17 compared to single CL, the Log Kb was 5.83. Binding results indicated that the DOX was more encapsulated in mixed LIB aggregates compared to single CL aggregates. This mixed aggregate LIB has excellent performance in controlling the release of drugs. For single CL aggregate, approximately 62.5% DOX was released after 72 h (pH = 7.4). However, LIB such as, (0.1αCL+ 0.9αIBF), the release of DOX decreased to 16.08%. Finally, LIB mixed aggregate applicability has been used to minimize the cytotoxicity of MCF-7 cells, and it has been found that DOX in LIB mixed aggregates has a higher inhibitory effect on cell growth than DOX in CL. [Display omitted] •Aggregation behavior of cationic lipid- ibuprofen mixtures has been evaluated.•Negative values of βm, ∆Gom and ∆Goex were indicated the synergistic interactions and spontaneity of the mixed aggregation.•Enhanced binding of DOX using cationic lipid- ibuprofen mixed aggregate•Encapsulation efficiency and controlled drug release enhanced at a high mole fraction of αIBF 0.9.•DOX in Cationic lipid- ibuprofen mixed aggregate has a higher inhibitory effect on cell growth than DOX in CL.