Voltammetric determination of uric acid using a miniaturized platform based on screen-printed electrodes modified with platinum nanoparticles

[Display omitted] •Disposable and sensitive SPCE based on PtNPs and MWCNT was designed to detect uric acid in biological fluids.•The approach has potential for POC analysis of urine samples.•Analysis developed with a miniaturized potentiostat coupled to a smartphone. Disposable and sensitive screen-printed carbon electrodes (SPCE) modified with multi-walled carbon nanotubes (MWCNT) and platinum nanoparticles (PtNPs) were constructed to analyse uric acid (UA) in biological samples using a miniaturized smartphone potentiostat system. Combining the nanomaterials with Nafion™ yielded a stable dispersion that could be successfully used as an electroactive layer. Transmission electron microscopy (TEM) and energy dispersive scanning (EDS) were used to characterize the morphology of PtNPs. These revealed approximately spherical shapes with emission lines characteristics of platinum. Furthermore, PtNPs/MWCNT/SPCE film was characterized by scanning electron microscopy (SEM), which demonstrated the tubular characteristic of MWCNT distributed and covered by carbon black (CB) spheres present in the screen-printed ink. An enhanced current response was observed for UA voltammetric analysis, especially due to the synergism effect of PtNPs and MWCNT, which reduced the Rct value. The values of Dapp (1.6 × 10−6 cm2/s) and kcat (2.76 × 103 mol−1 L/s) were determined for UA using the proposed sensor. The sensor displayed linear response for UA in the range from 5.0 × 10−6 mol/L to 6.9 × 10−4 mol/L, with a detection limit of 4.9 × 10−7 mol/L. Along with detectability, excellent performances were verified in terms of repeatability, anti-interference features, and analysis of UA in the biological samples.