Specific detection of sarin gas Mimicking, diethylchlorophosphate employing solvent mediated in-situ generated hydroxyl functional group in a phthalimide-chromone coupled fluorogenic probe

An exceptionally effective phthalimide-chromone coupled fluorogenic probe, CMAD has been introduced to detect sarin gas surrogate diethylchlorophosphate (DCP), both in solution and vapor phase, respectively, with quick response, excellent sensitivity, and specificity having detection and quantification limit in the μM range which is very lower than LD50 value. [Display omitted] •A phthalimide-based fluorogenic probe, CMAD has been introduced to detect sarin gas mimics diethylchlorophosphate (DCP).•CMAD is effective in detecting DCP in the solution and vapor phase, respectively.•The detection and quantification limit is in the μM range much lower than the LD50 value.•The demonstrations of practical usage, including soil analysis, inspire additional applications in different fields.•CMAD could be used in identifying DCP in the presence of other competing nerve agents. Nerve agents are particularly hazardous substances and can result in fatality due to suppressing acetylcholinesterase function. This article introduced a fluorescent probe, (E)-5-(((4-hydroxy-2-methoxy-2H-chromen-3-yl)methylene)amino)isoindoline-1,3-dione (CMAD), which was exceptionally effective at detecting the sarin replacement diethylchlorophosphate (DCP), both in solution and vapor phase, respectively which exhibited quick response, excellent sensitivity and specificity. As a result of the gradual addition of DCP into the solution of the probe, the photoluminescence intensity at 476 nm is markedly increased, exhibiting bright cyan color fluorescence because of inhibition of the excited state intramolecular proton transfer (ESIPT) process, which is also clearly reflected in the estimation of photoluminescence quantum yield of phosphorylated CMAD (0.55 relatives to 4-aminophalimide) and the color chromaticity (CIE) diagram. The lowest detection limit and limit of quantification are 3.25 µM and 10.8 µM, respectively, within the stockpiles of other analogous analytes. Additionally, for on-the-spot detection, the probe may also be utilized in the test strips with superior selectivity for DCP. The ability to identify and measure DCP in the gas phase has also been established using a dip-stick and dipped-vial-conical-flask analysis. Moreover, we also performed the smartphone-based technique for the practical utility of CMAD for the on-spot quantification and detection of sarin gas in real threat situations. The effective demonstrations of practical usage, including soil analysis, inspire addition