New perspective for probe development assessing salivary biomarkers in periodontal affected diabetic patients

[Display omitted] •Raman Spectroscopic probe as chair-side device for periodontal disease in diabetics.•MMP-8 and CTX can serve as valuable tool to assess periodontal disease.•Clinical and periodontal parameters worse among chronic diabetic moderate groups. The study was performed to have chairside identification of specific biomarkers for diagnosing periodontal disease in diabetic patients at a very early stage using spectroscopic probe design and parameters. Patients were divided into groups: Group (A) - healthy patients, Group (B) - patients with type 2 Diabetes Mellitus (DM) without chronic periodontitis, Group (C) - mild chronic periodontitis patients without type 2 DM, Group (D) - moderate chronic periodontitis patients without type 2 DM, Group (E) - mild chronic periodontitis patients with type 2 DM, and Group (F) - moderate chronic periodontitis patients with type 2 DM. Conceptual and practical design for achieving chairside Raman probe has specific tips and optics applied based on temperature of the mouth. Activation of Matrix metalloproteinase-8 (MMP-8) within salivary specimens were analysed. For molecular docking studies, binding pocket was selected with larger SiteScore. MMP-8 and C-terminal cross-linked telopeptide (CTX) of type I collagen levels in saliva were analysed using MMP-8 Immunoassay. Raman measurements were carried out of salivary specimens. Continuous variables were compared by one‑way analysis of variance. Rate of MMP-8/CTX release was significantly higher in diabetes chronic moderate periodontitis group (p