Firstly electrochemical examination of vildagliptin at disposable graphite sensor: Sensitive determination in drugs and human urine by square-wave voltammetry

[Display omitted] •PGE electrode was employed for the first time for determination of vildaglipitn.•Benefits of method: rapidity, simplicity, sensitivity and repeatability.•The procedure was successfully applied to vildagliptin detection in pharmaceutical and urine samples. The work describes the determination of vildagliptin at the pencil graphite electrode using cyclic voltammetry and square-wave voltammetry. Vildagliptin yielded a well-defined irreversible peak response in phosphate buffer (PBS 9.0) medium, at about +1.13 V by cyclic voltammetry. Using optimum square-wave voltammetry conditions, a good linear correlation was obtained between the anodic current signal and concentration, in the range of 2.94–49.98 µM in PBS (pH 9.0) medium, the limit of detection and relative standard deviation were calculated as 82 nM (24.88 ng L−1) and 2.95%, respectively. The developed voltammetric method was successfully applied for vildagliptin determination without any separation in pharmaceutical preparations and urine samples.