Non-enzymatic electrochemical sensor for the simultaneous determination of adenosine, adenine and uric acid in whole blood and urine

•For the first time, simultaneous electrochemical determination of adenosine, adenine and uric acid was manifested.•2-amino 5-mercapto 1,3,4-thiadiazole was used as the electrode modifier in phosphate buffer medium.•Viability of the sensor was demonstrated in blood and urine Table 3 samples. Herein we report for the first time, a simultaneous voltammetric sensor for adenosine, adenine and uric acid using a pencil graphite (PG) substrate electropolymerised with 2-amino-5-mercapto-1,3,4-thiadiazole in phosphate buffer with pH 7. The developed sensor was characterized morphologically, structurally and electrochemically. Under the optimum conditions, a linear concentration range of 0.2–25.6 µM, 0.05–12.8 µM and 5–1200 µM was obtained with a detection limit of 0.19 µM, 0.039 µM and 2.74 µM for adenosine, adenine and uric acid respectively. Viability of the sensor was also tested in blood and urine samples, and it was found that the sensor is an excellent tool for the simultaneous determination of the above mentioned targets. [Display omitted]